Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:37
FOS-Split Linker-Fok_a
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K243028_sequence (Version 1) |
Description
we fused Splitli-Fok_a with Fos, linked to an His-tag. In a mixture of the expressed protein with Jun and Fok_i and the target DNA simultaneously the dimerization of Fos-Splitli-Fok_a with Jun should lead to the interaction of Fos-Splitli-Fok_a with the DNA. After binding and hence activation of the Fok_i and Fok_a partners, the DNA should be cutted. As a prospect the additional feature of photo-switchability of Fos can be generated, allowing regulation of the enzyme???s activity. Introduction of cysteine residues as reactive sites into Fos and fusion of thiol-reactive cross-linkers to them results in intramolecular cross-linking of the Fos protein. By a photo-switch between cis and trans conformations these cross-linkers can change their end to end distance. Thus isomerization can be used to promote helix folding or unfolding of Fos (Woolley et al., Bioinformatics Advance Access published October 17, 2006).Notes
none.Source
Combined by serial cloning steps.| Access | Instance | Definition |
| public public public public public | BBa_K243027 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243000 | Fos Scar 25 linker Scar 25 Fok_a |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K243027 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243000 | 1,216 217,222 223,273 274,279 280,858 | Fos Scar 25 linker Scar 25 Fok_a |