Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Soufiane Boumahdi
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:41
OmpA_Linker + g3p
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K257016_sequence (Version 1) |
Description
This part allows us to send g3P to the outer membrane of the bacteria so that we can use g3P as an activator of the fusion between the vesicle and the recipient bacteria.Notes
In our project we use g3p as a fusion to OmpA-Linker (BBa_K103996) which need SacI restriction site for inframe fusion.- So we design g3p with SacI site at the N-terminal. SacI (GAGCT^C) site is shared with XbaI (T^CTAGA) in order to have SacI site for fusion and standard sites.
- Moreover we decide to suppress the signal peptide (18 first amino acids) which is cleaved in order to conserve the N-ter fusion.
Source
Composite of 2 de novo part.| Sequence Annotation | Location | Component / Role(s) |
| good rbs BBa_K257003 ompA Linker (partial) linker for C term fusion SacI restriction site BBa_K257001 N1 domain G1 (Gly-rich linker, EGGGS motif) N2 domain G2 (Gly-rich linker) CT domain Double stop codon | 1,8 1,473 14,448 449,474 469,474 474,1699 476,676 677,733 734,1126 1127,1243 1244,1693 1694,1699 | ribosome_entry_site feature/rbs feature/BioBrick engineered_region CDS feature/cds sequence_feature feature/misc feature/misc sequence_feature engineered_region feature/BioBrick sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature stop_codon feature/stop |