Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hikmet Geckil, Fulbright Postdoctoral Researcher
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:42
Vhb protein
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K258103_sequence (Version 1) |
Description
The gene coding for the Vitreoscilla hemoglobin (VHb) molecule has been cloned and functionally expressed in Escherichia coli. By using a plasmid-encoded gene as well as single-copy integrants, the oxygen-dependent VHb gene (VHb) promoter was shown to be functional in E. coli. The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation).We wanted to measure the activity of VHb gene; therefore, the VHb promoter. The VHb promoter becomes active when the surrounding oxygen amount lowers to 2%. We wanted to see whether this promoter will effectively work with our E. Coli cells.
Notes
no any considerationSource
gene coding for the Vitreoscilla hemoglobin (VHb)| Access | Instance | Definition |
| public public public public public | BBa_K258005 BBa_B0034 BBa_K258014 BBa_B0010 BBa_B0012 | BBa_K258005 BBa_B0034 BBa_K258014 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K258005 BBa_B0034 BBa_K258014 BBa_B0010 BBa_B0012 | 1,137 146,157 164,601 610,689 698,738 | BBa_K258005 BBa_B0034 BBa_K258014 BBa_B0010 BBa_B0012 |