BBa_K259006

BBa_K259006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K259006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-09-08 11:00:00
Date modified: 2015-05-08 01:11:42

GFP-Terminator



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K259006_sequence (Version 1)

Description

Part Description



This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.

Green Fluorescent protein (originally from part BBa_E0040) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene.
This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.

Table of Statistics


{| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2"
!Chassis
!Monomer/Multimer
!Aminoacid Length/Weight
!Localisation
!Structure
!Excitation wavelengths
!Emmision wavelengths
|-
|Prokaryotic and Eukaryotic
|Bipartite
|274 aminoacids
|
|beta-barrel(for GFP) hairpins for Terminator
|501nm
|511nm
|}



References



#Valdivia pmid=8707053


Notes

No additional design considerations. The parts were fused together following RFC10 assembly.

Source

GFP originates from jellyfish Aequeora victoria. For more details visit the SwissProt entry.

The double terminator was designed by Reshma Shetty. For more details visis the original part page.

Access Instance Definition
public
public
public
BBa_E0040
BBa_B0012
BBa_B0011
GFP
BBa_B0012
BBa_B0011
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K259006/1