Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42
pMA-RQ-@TetFlp
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K260009_sequence (Version 1) |
Description
This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp ([[Part:BBa_K260002|]]) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone.This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|]]). The product of the above-described recombineering reaction [[Part:BBa_K260002|]]-[[Part:BBa_K260017|]] is also available.
Notes
The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone pMA-RQ-@TetFlp to pTetFlp [[Part:BBa_K260002|]] via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.Source
Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|]].| Sequence Annotation | Location | Component / Role(s) |
| BBa_B1006 (terminator) suffix BBa_K260002 5' homology BBa_K260002 3' homology ColE1 origin AmpR PvuII site PvuII site prefix | 22,60 1,21 67,136 2502,2571 317,984 1132,1992 137,142 2496,2501 2578,2599 | feature/stem_loop stem_loop feature/BioBrick engineered_region sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds feature/dna sequence_feature sequence_feature feature/dna feature/BioBrick engineered_region |