Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Charoy
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:49
pLac (LacI regulated) + Strong RBS
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K292002_sequence (Version 1) |
Description
This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI.We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle.
In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
This part already contains a strong RBS to begin directly a gene transcription.
Notes
N/ASource
Part:BBa_R0010Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.