BBa_K300008

BBa_K300008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K300008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi

Date created: 2010-10-04 11:00:00

Date modified: 2015-05-08 01:11:51

Cm resistance and R6K replication origin

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K300008_sequence (Version 1)

Description

This part contains a Chloramphenicol resistance cassette and the R6K conditional replication origin.

When this part is contained in a pSB*** plasmid, it can be propagated in all E. coli strains. However, when this part is cut with XbaI-SpeI and self-ligated, it can be propagated only in pir+ abd pir116 E. coli strains.

This part has been used to construct BBa_K300000 integrative vector.

Notes

We used BioBrick Standard Assembly.

Source

- 2009 DNA Distribution;

- 2009 DNA Distribution;

- 2010 DNA Distribution.

Components

Access	Instance	Definition
public	BBa_P1004	cmR
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_J61001	R6K

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_P1004	1,769	cmR
BBa_B0010	778,857	BBa_B0010
BBa_B0012	866,906	BBa_B0012
BBa_J61001	915,1320	R6K

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K300008/1

Member of these Collections

• iGEM Parts Registry