3

BBa_K316010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-21 11:00:00

Date modified: 2015-05-08 01:11:56

Reverse strand coding XylE under LacI activation

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K316010_sequence (Version 1)

Description

This part contains XylE under synthetic hyperspank promoter BBa_K143000 LacI and IPTG are required for repression and activation of expression. As transformation of B subtilis often requires genomic integration, read-through from upstream genomic regions can become an issue. For the purposes of characterisation, the part was made on the minus strand to reduce background transcription. Read-through may provide an additional benefit as low quantities of anti-sence RNA may reduce basal expression sometimes seen in LacI systems. (although there is some evidence background is usually due to readthrough if have ref)

Notes

to obtain a 3' coding segment a number of PRC steps were required:

Source

Biobricks BBa_K143000 and BBa_J33204. The part contains a synthetic RBS optimised for high expression in B subtilis, calculated using RBS calculator http://voigtlab.ucsf.edu/software/

Components

Access	Instance	Definition
public	BBa_K316011	BBa_K316011
public	BBa_K316000	sRBS Pehs

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K316011	1,924	BBa_K316011
BBa_K316000	933,1081	sRBS Pehs

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316010/1

Member of these Collections

• iGEM Parts Registry