BBa_K316014

BBa_K316014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-22 11:00:00

Date modified: 2015-05-08 01:11:56

Dif sequence followed by PmeI recognition site

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K316014_sequence (Version 1)

Description

This composite part of BBa_K143000 and BBa_K143013. The dif site can be used in conjunction with another dif site in another part of the vector to remove a sequence between the two dif sites. PmeI site can be used for blunt end cloning of a DNA sequence behind the dif site.

Please see ???Part Design??? section for design considerations and parts used.

Notes

This part was designed to be cloned using standard biobrick methods. Two single stranded, synthetic oligos were annealed to produce a double stranded DNA sequence with single stranded overhangs identical to the product of digestion by EcoRI and SpeI. Thus compatible with biobrick cloning methods.

Source

Oligonucleotide synthesis of single stranded primers.

Components

Access	Instance	Definition
public	BBa_K316002	Bs dif
public	BBa_K316013	PmeI site

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K316002	1,28	Bs dif
BBa_K316013	37,44	PmeI site

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316014/1

Member of these Collections

• iGEM Parts Registry