BBa_K316023

BBa_K316023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-22 11:00:00

Date modified: 2015-05-08 01:11:56

Chloraphenicol resistance gene with 5amyE

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K316023_sequence (Version 1)

Description

BBa_K143001 integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence BBa_K143002 is also required.

This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce BBa_K143002.

Notes

Parts combined by standard biobrick assembly http://partsregistry.org/Assembly:Standard_assembly

Source

iobrick parts BBa_K143070 (which contains BBa_K143001, BBa_K316012, BBa_K143021) and BBa_K143064 (which contains BBa_K143031, BBa_B0015)

Components

Access	Instance	Definition
public	BBa_K143070	BBa_K143070
public	BBa_K143064	CmR-T

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143070	1,647	BBa_K143070
BBa_K143064	654,1450	CmR-T

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316023/1

Member of these Collections

• iGEM Parts Registry