BBa_K316031

BBa_K316031 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316031

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-19 11:00:00

Date modified: 2015-05-08 01:11:57

LytC cell wall binding domain with Pveg promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K316031_sequence (Version 1)

Description

We designed a protein that carries our signal peptide ComC out of the cell where a protease has access to it. The protease can then proceed to cleave ComC off the protein, allowing quorum sensing via the the ComCDE system to take place. One big problem we have to overcome is the cell wall that will obstruct the protease???s access to the protein carrying the signal peptide. The Detection module consists of a cell wall anchor, a signal peptide called ComC, and a linker that connects the two and is specifically designed to be cleaved by the protease we want to detect

Notes

Pfu DNA polymerase was used to minimise PCR errors

Source

Genome PCR from B. subtilis genome (strain) with linker-TEV cleavable site-ComC synthesised and attached via a PME site within LytC gene

Components

Access	Instance	Definition
public	BBa_K316001	Pveg
public	BBa_K316030	BBa_K316030

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K316001	1,97	Pveg
BBa_K316030	106,1079	BBa_K316030

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316031/1

Member of these Collections

• iGEM Parts Registry