BBa_K316031

BBa_K316031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:11:57

LytC cell wall binding domain with Pveg promoter



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K316031_sequence (Version 1)

Description

We designed a protein that carries our signal peptide ComC out of the cell where a protease has access to it. The protease can then proceed to cleave ComC off the protein, allowing quorum sensing via the the ComCDE system to take place. One big problem we have to overcome is the cell wall that will obstruct the protease???s access to the protein carrying the signal peptide. The Detection module consists of a cell wall anchor, a signal peptide called ComC, and a linker that connects the two and is specifically designed to be cleaved by the protease we want to detect

Notes

Pfu DNA polymerase was used to minimise PCR errors

Source

Genome PCR from B. subtilis genome (strain) with linker-TEV cleavable site-ComC synthesised and attached via a PME site within LytC gene

Access Instance Definition
public
public
BBa_K316001
BBa_K316030
Pveg
BBa_K316030
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K316031/1