BBa_K318013

BBa_K318013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K318013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathaniel Pantalone, Justin Vrana
Date created: 2010-07-12 11:00:00
Date modified: 2015-05-08 01:11:57

T + pLac + RBS + Int (P22) + T



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K318013_sequence (Version 1)

Description

This composite part is an inducible P22 Int integrase generator which acts on the P22 attB and attP recombination sites. It is composed of the following biobricks: BBa_B1006, BBa_K200021, BBa_I11030, BBa_B1006.

Notes

To minimize potential transcription due to upstream promoter activity, a terminator was placed upstream of the pLac promoter. A terminator was placed downstream of the Int encoding sequence to end transcription after the recombinase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Source

Biobricks

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K318013/1