BBa_K318013

BBa_K318013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K318013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathaniel Pantalone, Justin Vrana

Date created: 2010-07-12 11:00:00

Date modified: 2015-05-08 01:11:57

T + pLac + RBS + Int (P22) + T

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K318013_sequence (Version 1)

Description

This composite part is an inducible P22 Int integrase generator which acts on the P22 attB and attP recombination sites. It is composed of the following biobricks: BBa_B1006, BBa_K200021, BBa_I11030, BBa_B1006.

Notes

To minimize potential transcription due to upstream promoter activity, a terminator was placed upstream of the pLac promoter. A terminator was placed downstream of the Int encoding sequence to end transcription after the recombinase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Source

Biobricks

Components

Access	Instance	Definition
public	BBa_B1006	BBa_B1006
public	BBa_K200021	BBa_K200021
public	BBa_I11030	BBa_I11030
public	BBa_B1006	BBa_B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B1006	1,39	BBa_B1006
BBa_K200021	48,122	BBa_K200021
BBa_I11030	129,1353	BBa_I11030
BBa_B1006	1362,1400	BBa_B1006

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K318013/1

Member of these Collections

• iGEM Parts Registry