BBa_K319043

BBa_K319043 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K319043

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Afnan Azizi

Date created: 2010-10-25 11:00:00

Date modified: 2015-05-08 01:11:59

ADE4 targeting vector

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K319043_sequence (Version 1)

Description

This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.

Notes

N/A

Source

n

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EcoRI/MfeI mixed site	1,6	feature/misc, sequence_feature
ATM1 terminator	7,206	feature/misc, sequence_feature
EcoRI	227,232	feature/misc, sequence_feature
G5C5G5 filler site	233,247	feature/misc, sequence_feature
PstI site	248,253	feature/misc, sequence_feature
Forward landing pad	207,226	primer_binding_site, feature/primer_binding
Reverse landing pad	254,273	feature/primer_binding, primer_binding_site
ADE4 terminator	274,473	sequence_feature, feature/misc
PstI/NsiI mixed site	474,479	feature/misc, sequence_feature
Cm resistance	1620,2279	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K319043/1

Member of these Collections

• //chassis/eukaryote/yeast
• //classic/plasmid/uncategorized
• //plasmid/chromosomalintegration
• //plasmid/construction
• iGEM Parts Registry
• igem_plasmid_vectors