Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Meng Lu and William Rostain
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:11:59
Green light sensor with LacZ reporter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K322120_sequence (Version 1) |
Description
This design involves the fusion of CcaS and PhoR to create a green light sensor, by replacing the downstream portion of the CcaS with the downstream portion of PhoR based on the fact that both contain the same domains HisKA and HATPase-C. PhoR phosphorylates the response regulator PhoB, which then will binds to conserved DNA sequences called phoboxes and interacts with the &70 subunit of RNA polymerase to control transcription. Thus, we can add the reporter gene here as a response to the detection of green light.Notes
CcaS and PhoR combine to be the sensor biobrick and PhoR and LacZ combine to be the biobrick of reporter part.Source
CcaS: Synechocystis sp. (strain PCC 6803)- Synthesised by Unam Mexico 2010PhoR: Escherichia coli (strain K12)genomic DNA
PhoA promoter: Escherichia coli K12
LacZ reporter: Escherichia coli K12
| Access | Instance | Definition |
| public public public | BBa_K322123 BBa_K322119 BBa_K322117 | BBa_K322123 BBa_K322119 BBa_K322117 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K322123 BBa_K322119 BBa_K322117 | 1,1399 1406,3721 3730,4140 | BBa_K322123 BBa_K322119 BBa_K322117 |