Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Rostain
Date created: 2010-10-07 11:00:00
Date modified: 2015-05-08 01:12:00
Lac promoter + S284T firefly luciferase mutant (red colour)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K322247_sequence (Version 1) |
Description
This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red.Note that firefly luciferase is temperature sensitive, and cells grown at 30??C are considerably brighter than cells grown at 37??C.
Notes
Note that there is a LacZ fragment between the lac promoter and the luciferase translational unit.Also, the luciferase is temperature sensitive and growing the cells at 30??C will increase brightness.
Source
From BBa_I712019, then mutated using the MABEL protocol (see French lab on openwetware).| Access | Instance | Definition |
| public public public | BBa_J33207 BBa_J15001 BBa_K322246 | BBa_J33207 BBa_J15001 BBa_K322246 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_J15001 BBa_K322246 | 1,600 609,618 625,2277 | BBa_J33207 BBa_J15001 BBa_K322246 |