Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julius Ho
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02
TetR repressible generator of LacI
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K324003_sequence (Version 1) |
Description
This part combines two biobricks, a TetR repressible promoter+RBS (BBa_J13002) and the LacI regulatory protein in RFC25 assembly (BBa_K324000). This BioBrick allows the expression of LacI when inserted into E. coli, for the purpose of testing constructs controlled by the LacI promoter.Notes
These parts were joined together with standard biobrick assembly, even though the LacI protein was in RFC25 and thus capable of protein fusion assembly as well.Source
A simple Biobrick assembly was conducted to join the TetR promoter+RBS (BBa_J13002) with LacI (BBa_K324000). J13002 was obtained from the 2010 Spring Distribution, while K324000 was adapted by 2010 Brown iGEM from an existing part. See the part's page for further detailshttp://partsregistry.org/Part:BBa_J13002
http://partsregistry.org/Part:BBa_K324000
| Access | Instance | Definition |
| public public | BBa_J13002 BBa_K324000 | BBa_J13002 BBa_K324000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J13002 BBa_K324000 | 1,74 81,1184 | BBa_J13002 BBa_K324000 |