Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ethan Richman
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02
TrpR-RBS-TetR-Term-pTetR-RBS-CI-RBS-AraC-RBS-Mnt-Term
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K324050_sequence (Version 1) |
Description
This part is a double repressor system derived from [[Part:BBa_K191005]]. A Trp promotor controls expression of tetR, a repressor to the Tet promotor. The Trp promotor is ON, unless otherwise repressed. The expression of CI, AraC, and Mnt, which follow the Tet promotor, is inhibited given the absence of tryptophan.Usage
This part is designed to interact with [[Part:BBa_K191003]], as the light-activated LovTAP binds to and represses the Trp promotor. Thus, given additional expression of LovTAP, the presence of light causes the repression of TetR expression and the activation of expression of CI, AraC, and Mnt. You can see the full circuit design below - press the light button to toggle the circuit. [[Image:LightCircuit.swf]].
Notes
Because there are illegal SpeI sites in the Trp promotor, all assembly had to be done with RFC 21 standard (bgl bricks). Thus, we had to add the appropriate bgl-brick sites to all parts we worked with. See [[Team:Brown/Project/LRC_overview]] for a more in depth description.Source
[[Part:BBa_K191005]] for TrpR-RBS-TetR-Term-pTetR (PCR to isolate this segment)[[Part:BBa_B0034]] for sequence of RBS (added RBS by PCR to CI, AraC, and Mnt)