BBa_K324050

BBa_K324050 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K324050
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ethan Richman
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02

TrpR-RBS-TetR-Term-pTetR-RBS-CI-RBS-AraC-RBS-Mnt-Term



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K324050_sequence (Version 1)

Description

This part is a double repressor system derived from [[Part:BBa_K191005]]. A Trp promotor controls expression of tetR, a repressor to the Tet promotor. The Trp promotor is ON, unless otherwise repressed. The expression of CI, AraC, and Mnt, which follow the Tet promotor, is inhibited given the absence of tryptophan.

Usage


This part is designed to interact with [[Part:BBa_K191003]], as the light-activated LovTAP binds to and represses the Trp promotor. Thus, given additional expression of LovTAP, the presence of light causes the repression of TetR expression and the activation of expression of CI, AraC, and Mnt. You can see the full circuit design below - press the light button to toggle the circuit. [[Image:LightCircuit.swf]].

Notes

Because there are illegal SpeI sites in the Trp promotor, all assembly had to be done with RFC 21 standard (bgl bricks). Thus, we had to add the appropriate bgl-brick sites to all parts we worked with. See [[Team:Brown/Project/LRC_overview]] for a more in depth description.

Source

[[Part:BBa_K191005]] for TrpR-RBS-TetR-Term-pTetR (PCR to isolate this segment)
[[Part:BBa_B0034]] for sequence of RBS (added RBS by PCR to CI, AraC, and Mnt)

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K324050/1