BBa_K324051

BBa_K324051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K324051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ethan Richman

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:02

LacI RFP Bistable part GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K324051_sequence (Version 1)

Description

This is the Bistable part originally created by team PKU '07, modified by PKU '09, and now further modified by us. We have added expression of LacI before RFP and the CI434 regulated promotor. As described in BBa_K2280034, and detailed here this part has two stable states. Initially, the green, or CI434 state dominates. However, as CI is introduced, expression of CI434 (and thus state 1) is inhibited, and the bistable part switches to state 2, where CI and RFP are produced. We have added the additional expression of LacI in state 2.

Notes

See our project page for detailed design considerations.

Source

BBa_K2280034 for Bistable part
BBa_C0021 for LacI
http://parts.mit.edu/igem07/index.php/Peking_Push-on-push-off#Constructing_the_Bistable_Switch

Components

Access	Instance	Definition
public	BBa_C0012	lacI
public	BBa_K228003	BBa_K228003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_C0012	1,1128	lacI
BBa_K228003	1162,4099	BBa_K228003

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K324051/1

Member of these Collections

• iGEM Parts Registry