BBa_K336053

BBa_K336053 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K336053

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Naoto Shimada

Date created: 2010-10-26 11:00:00

Date modified: 2015-05-08 01:12:05

Over expression system (T7 polymerase induced by lac promoter)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K336053_sequence (Version 1)

Description

To obtain large amount of protein, over expression system was designed.
Expression of T7 polymerase[http://partsregistry.org/Part:BBa_K145001]is induced by IPTG.
Add IPTG when you need, you obtain target of protein at appropriate　phase.

Notes

only design

Source

R0010:promoter (lacI regulated)
B0034:RBS (Elowitz 1999) -- defines RBS efficiency
K145001:T7 RNA polymerase
B0015:double terminator (B0010-B0012)

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K145001	T7pol
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K145001	227,2881	T7pol
BBa_B0015	2890,3018	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K336053/1

Member of these Collections

• iGEM Parts Registry