fecA-prhA

BBa_K367006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K367006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fernando Govantes

Date created: 2010-06-29 11:00:00

Date modified: 2015-05-08 01:12:15

fecA-prhA fusion coding sequence

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K367006_sequence (Version 1)

Description

Artificial coding sequence spanning the first 92 codons of fecA, encoding the signal peptide (aa. 1-33), the proposed Ton-box (aa. 54-63), fused to the distal end of the prhA coding sequence at the conserved GSGL motif (aa. 89-92).

Notes

No edits other than the fusion junction, and the introduction of the standard biobrick stop codons TAATAA

Source

fecA sequence comes from Escherichia coli str. K-12 substr. MG1655, complete genome.
GenBank: U00096.2

prhA sequence comes from Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence. NCBI Reference Sequence: NC_003296.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Fused fecA-prhA coding sequence	1,2286	CDS, feature/cds
fecA start codon	1,3	start_codon, feature/start
Encodes FecA signal peptide	1,99	sequence_feature, feature/misc
Encodes FecA N-terminal extension domain	100,276	feature/protein, CDS
Encodes conserved GSGL motif, common to FecA and PrhA	265,276	conserved_region, feature/conserved
Encodes PrhA C-terminus, starting at amino acid 97	265,2286	CDS, feature/protein
Standard biobrick double stop codon	2287,2292	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K367006/1

Member of these Collections

• iGEM Parts Registry