BBa_K368009

BBa_K368009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K368009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2010-10-23 11:00:00

Date modified: 2015-05-08 01:12:15

tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K368009_sequence (Version 1)

Description

eukaryots:
tet-on Promoter: doxycycline inducible CMV promoter (special cell line needed!)
TEV recogn.site: specific restriction site for TEV protease
N-degron: degradation signal for proteins if N-terminus is free
SF3b155: Splicing factor 3b, size: 155kDa, interacting with p14*
Human Bak: mitochondrial membrane protein, causing apoptosis if overexpressed
SV40PA: polyadenylation signal, needed for translation in eukaryots

Notes

-> "Cut'N'survive" system

Source

(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))

Components

Access	Instance	Definition
public	BBa_K368007	BBa_K368007
public	BBa_K368002	BBa_K368002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K368007	1,1635	BBa_K368007
BBa_K368002	1642,2485	BBa_K368002

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K368009/1

Member of these Collections

• iGEM Parts Registry