View

BBa_K389050

BBa_K389050 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K389050

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonas Aretz

Date created: 2010-09-27 11:00:00

Date modified: 2015-05-08 01:12:19

Tightly controlled lac operon

Back

Details

Types	DnaRegion
Roles	Regulatory promoter
Sequences	BBa_K389050_sequence (Version 1)

Description

This BioBrick is a tightly controlled lac operon. It contains a lacIq and a lac operator, so it is inducible with IPTG but has a low basal expression. It is also possible to use this BioBrick in strains that do not carry a lacIq like E. coli TOP10.

Notes

we needed a tightly controlled inducible promoter

Source

partsregistry

Components

Access	Instance	Definition
public public public public public	BBa_I14032 BBa_B0034 BBa_C0012 BBa_B0017 BBa_R0010	P(Lac) IQ BBa_B0034 lacI BBa_B0017 LacI

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032 BBa_B0034 BBa_C0012 BBa_B0017 BBa_R0010	1,37 46,57 64,1191 1225,1392 1401,1600	P(Lac) IQ BBa_B0034 lacI BBa_B0017 LacI

Other Properties

igem#experience	Fails
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K389050/1