Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ling Li
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:22
GFP pulse generator with pLux/cI434 hybrid promoter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K396006_sequence (Version 1) |
Description
When there is AHL induction, GFP generats as a pulse because of cI434 repression.Notes
hybrid promoter has lux box in its upstream and cI434 operation site(OR1,OR2)in its downstream. The RBS of cI434 is weak and GFP is designed destabilized with LVA tag.Source
Ron Weiss. Spatiotemporal control of gene expression with pulse-generating networks| Sequence Annotation | Location | Component / Role(s) |
| BBa_K396001 GFPuv BBa_K396002 LVA cI434 OR1 -10 cI434 OR2 -35 Lux Box BBa_R1062 LuxR/HSL -35 -10 BBa_B0031 cI 434 BBa_C0052 LVA RBS(weak) | 1,839 1,756 1,61 4,36 779,793 793,798 800,814 815,820 819,839 854,909 854,873 874,879 895,901 910,923 930,1595 930,1595 1560,1592 763,769 | engineered_region feature/BioBrick CDS feature/protein feature/BioBrick engineered_region tag feature/tag operator feature/operator feature/promoter promoter feature/operator operator promoter feature/promoter non_covalent_binding_site feature/binding engineered_region feature/BioBrick feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter feature/BioBrick engineered_region feature/cds CDS feature/BioBrick engineered_region feature/tag tag feature/rbs ribosome_entry_site |