Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonghwa Kim
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:23
composite heavy metal-detector(Zn, Cd, As)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K400000_sequence (Version 1) |
Description
It is a composite part of heavy metal-detecting promoters and reporter genes.----PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----
It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color.
This is our final product to put in a capsule in order to be customized.
Notes
Each of the parts were cleaved with Endonuclease and ligated with pSB1C3 vector.First, a promoter and a reporter gene were amplified by PCR and two of the pairs were ligated with pSB1C3 vector.
Second, another promoter and reporter gene were put together by PCR and then ligated with pSB1C3 vector with the other two-pair-fragment.
Three pairs were finally put in a vector and it underwent point mutation during the procedure because it had multiple cleavage sites.
Source
a soil bacterium| Sequence Annotation | Location | Component / Role(s) |
| GFP protein BBa_E0040 BBa_R0010 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J04450 mutated aryl acylamidase BBa_K400628 | 1,720 1,720 729,928 817,854 865,870 889,894 894,928 901,901 937,948 941,944 955,1635 955,1629 1636,1660 1669,1748 1680,1723 1757,1797 1764,1783 1784,1797 1790,1790 729,1797 1804,3297 1804,3297 | feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter non_covalent_binding_site feature/binding start_codon feature/start engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick feature/cds CDS feature/barcode engineered_tag feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region CDS feature/protein engineered_region feature/BioBrick |