Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristina Ehrhardt
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:12:26
PyeaR+RFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K412001_sequence (Version 1) |
Description
PyeaR+RFP is a nitrate/nitrite detector. PyeaR (BBa_K216005) is a nitrate/nitrite inducible promoter, that we ligated to RFP (K093005), so that we could detect nitrates and nitrites.Notes
We knew we wanted a nitrate sensitive promoter followed by a reporter to work in E.coli. Therefore we digested PyeaR with SpeI and PstI and digested RFP with XbaI and PstI and then ligated the two parts together to form this composite part.Source
We got both subparts from the registry, when we looked for a nitrate detector and a reporter.| Access | Instance | Definition |
| public public | BBa_K216005 BBa_K093005 | BBa_K216005 RBS-RFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K216005 BBa_K093005 | 1,100 109,832 | BBa_K216005 RBS-RFP |