Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Polasko
Date created: 2010-10-17 11:00:00
Date modified: 2015-05-08 01:12:26
35S Promoter + (Strong Plant RBS + GFP) From K414001
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K414006_sequence (Version 1) |
Description
This composite was designed to bring together a constitutive plant promoter (35S) with a fluorescent reporter (GFP) that has a strong plant RBS (Kozak)upstream to maximize translational efficiency. A composite part like this one may be used as an internal control in plant transformations to measure relative, induced expression of an inducible gene.Notes
No additional design considerations. Standard part assembly protocol followed.Source
This part was made with the 35S from BBa_K414002 (Wheeler, 2010) who obtained it from pK7FWG2 plasmid. Also the GFP with strong plant RBS was taken from BBa_K414001 (Bersaba, 2010).| Access | Instance | Definition |
| public public | BBa_K414002 BBa_K414001 | BBa_K414002 BBa_K414001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K414002 BBa_K414001 | 1,1029 1038,1775 | BBa_K414002 BBa_K414001 |