Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shawn Pan
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:27
Cm Backbone with pBR322 Derived Origin
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K415049_sequence (Version 1) |
Description
This part is a biobrick backbone with chloramphenicol resistance and pBR322 derived origin. The copy number is expected to be around 20. The characterization of this part is currently happening, and more data will be uploaded soon.Note: The backbone contains an extra VR sequencing site from the origin K415039. This site will be removed in the final version pSB6C5. Also, cells containing this plasmid grow slower than usual.
Notes
The extra reverse sequencing site needs to be removed in the final version (pSB6C5). The resistance P1004 was placed before the origin operon K415039 to provide a promoter to the copy-number regulating protein ROP.Source
This backbone was constructed with the standard biobrick plasmid construction method. The origin is K415039 and the chloramphenicol resistance is from P1004.After several failures in getting the correct colonies with the ccdB containing base vector I51020, the appropriate base vector region was obtained by PCR of an existing version 5 backbone containing RFP (pSB3T5). The RFP version of the base vector allowed for easy screening for correct colonies.
| Sequence Annotation | Location | Component / Role(s) |
| Biobrick Prefix Biobrick Suffix Extra VR site VR Site VF2 Site P1004 (Cm resistance) pMB1 Origin ROP RBS | 2405,2426 1,21 1479,1498 116,135 2281,2301 184,952 1583,2197 979,1172 969,974 | feature/misc sequence_feature feature/misc sequence_feature promoter feature/promoter promoter feature/promoter promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/rbs ribosome_entry_site |