Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-08-19 11:00:00
Date modified: 2015-05-08 01:12:27
J23117 : B0031 : cymR Repressor Protein [Weak cymR Generator]
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K415205_sequence (Version 1) |
Description
This is a copy of a moderately expressed cymR repressor under the control of a weak,constitutive promoter. This is a modular component for circuit construction and a weak
cymR generator.
Notes
Note that a weak promoter was chosen in order to follow the construction design of Choi, etal. in the 2010 paper, "A novel, versatile, and tightly regulated expression system for E. Coli
strains." Previous work by Choi and others in M. Extorquens indicated that overexpression
of cymR was potentially toxic to bacterial cells; although data concerning this toxicity is
unavailable, the researchers managed to develop a tightly expressed cymR switch by placing
cymR under the control of a weak, constitutive promoter for kanamycin resistance. As our
team was unable to clarify the origin of this sequence, we instead selected a weak, constitutive
promoter from the Anderson library.
Source
This part consists of BioBricks BBa_J23117 and BBa_K415203.| Access | Instance | Definition |
| public public public | BBa_J23117 BBa_B0031 BBa_K415202 | BBa_J23117 BBa_B0031 BBa_K415202 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23117 BBa_B0031 BBa_K415202 | 1,35 44,57 64,675 | BBa_J23117 BBa_B0031 BBa_K415202 |