Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Russell Durrett
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:27
Aga2 fused to eCFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K416008_sequence (Version 1) |
Description
When working correctly this reporter construct transcribes Aga2-eCFP from the Gal promoter. If the yeast strain is also expressing Aga1, then this fusion should be anchored onto the plasma membrane, allowing you to visualize eCFP as a halo around the yeast cell.Notes
We considered fusing this reporter to a variety of promoters, but in the end chose pGAL because it gives a very high level of transcription and we could turn the reporter on and offSource
This part comes from a mixture of biobricks that were received in the 2010 initial distribution and from parts that NYU iGEM 2010 made ourselves.| Access | Instance | Definition |
| public public public public public | BBa_K416005 BBa_K416000 BBa_K416001 BBa_K105009 BBa_K106018 | BBa_K416005 BBa_K416000 BBa_K416001 ECFP BBa_K106018 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K416005 BBa_K416000 BBa_K416001 BBa_K105009 BBa_K106018 | 1,575 582,842 851,895 904,1647 1656,1835 | BBa_K416005 BBa_K416000 BBa_K416001 ECFP BBa_K106018 |