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BBa_K429000

BBa_K429000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K429000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefano Fenu

Date created: 2010-10-25 11:00:00

Date modified: 2015-05-08 01:12:29

Promoter Testing Construct

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K429000_sequence (Version 1)

Description

This part is designed to act as a testing chasis for new promoters. A promoter can be extended from the B0034 end of the part by PCR extension, and if promoter activity is repressed GFP will be expressed.

Notes

Note: The assembly and transformation of the part can initially be tested by the induction of GFP expression in the presence of tetracycline or anhydrotetracycline.

Source

This is a composite of BBa_Q04400, and BBa_I13504.

Components

Access	Instance	Definition
public public public public public public public public public	BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,12 19,703 712,791 800,840 849,902 911,922 929,1648 1657,1736 1745,1785	BBa_B0034 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K429000/1