Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero & Fernando Govantes
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-tetR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510017_sequence (Version 1) |
Description
The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a tetR/ptetR cassette (BBa_I732083) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism.Notes
The BBa_I732083 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.Source
The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.| Access | Instance | Definition |
| public public | BBa_K510012 BBa_I732083 | BBa_K510012 BBa_I732083 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_I732083 | 1,4549 4558,5446 | BBa_K510012 BBa_I732083 |