Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victor Alvarez, Felix Reyes and Yolanda Gonzalez
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30
Improved Flip Flop (Module I)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K510019_sequence (Version 1) |
Description
Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure.Source
It's a part which comes from different genes forming the bassic flip-flop with anothers improvements.| Sequence Annotation | Location | Component / Role(s) |
| Double terminator Restriction Site: AflII Restriction Site: AscI GFP::Das4 DAS4 RBS Restriction Site: BamHI LacI::Das4 DAS4 RBS PcI Restriction Site: Sma I PLac RBS cI (Ts) Restriction Site: NheI RBS+OmpN::SspB RBS+OmpN Restriction Site: SacII RBS+OmpN RBS+OmpN::RFP Restriction Site: StuI Double terminator | 10,138 139,144 147,154 155,919 161,205 926,938 940,945 947,2077 953,997 2084,2096 2105,2153 2171,2176 2187,2386 2395,2407 2414,3127 3130,3135 3139,3732 3139,3234 3735,3740 3742,3837 3742,4518 4526,4531 4534,4662 | stop_codon feature/stop feature/dna sequence_feature sequence_feature feature/dna CDS feature/cds feature/tag tag ribosome_entry_site feature/rbs sequence_feature feature/dna feature/cds CDS tag feature/tag ribosome_entry_site feature/rbs promoter feature/promoter sequence_feature feature/dna promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/cds sequence_feature feature/dna feature/cds CDS feature/misc sequence_feature sequence_feature feature/dna feature/misc sequence_feature CDS feature/cds sequence_feature feature/dna stop_codon feature/stop |