Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero & Fernando Govantes
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-Lux
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K510021_sequence (Version 1) |
Description
The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.Notes
The BBa_K325909 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.Source
The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.| Access | Instance | Definition |
| public public | BBa_K510012 BBa_K325909 | BBa_K510012 BBa_K325909 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_K325909 | 1,4549 4558,12194 | BBa_K510012 BBa_K325909 |