Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K511910_sequence (Version 1) |
Description
This MammoBlock composite device produces the LacI-KRAB strong repressor protein when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.Source
Consult constituent components.| Access | Instance | Definition |
| public public public | BBa_K415506 BBa_J85600 BBa_K511205 | BBa_K415506 BBa_J85600 BBa_K511205 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K415506 BBa_J85600 BBa_K511205 | 1,330 331,352 353,1819 | BBa_K415506 BBa_J85600 BBa_K511205 |