BBa_K518006

BBa_K518006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K518006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Masato Ohgishi

Date created: 2011-09-14 11:00:00

Date modified: 2015-05-08 01:12:33

IPTG-inducible CheZ expression device

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K518006_sequence (Version 1)

Description

CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device rescues cheZ-/- cells in the presence of IPTG.

Notes

None.

Source

cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.

Components

Access	Instance	Definition
public	BBa_R0011	lacI+pL
public	BBa_B0032	BBa_B0032
public	BBa_K518005	BBa_K518005
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011	1,54	lacI+pL
BBa_B0032	64,76	BBa_B0032
BBa_K518005	85,889	BBa_K518005
BBa_B0014	898,992	BBa_B0014

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K518006/1

Member of these Collections

• //function/motility
• iGEM Parts Registry