Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-07-18 11:00:00
Date modified: 2015-05-08 01:12:33
RBS + bglX (E. coli perisplasmic β
-glucosidase)
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K523002_sequence (Version 1) |
Description
This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.The part was made using the strategy outlined in
Notes
The part was made using the strategy outlined inThe part was created from a lab E. coli strain using primers:
*Forward: ACT AGATCT gcc acg tcg ggc aac
**adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of
*Reverse: ACT ACTAGT A TTA tta cag caa ctc aaa ctc gcc
**adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon.
Source
The source E. coli genome sequence can be seen using GenBank U00096.2| Sequence Annotation | Location | Component / Role(s) |
| BglII Native RBS bglX ATG TAATAA PstI site removed G to T silent mutation Predicted (cleaved) signal peptide | 1,4 22,25 38,2338 38,38 2333,2333 85,85 2122,2122 38,97 | feature/misc sequence_feature sequence_feature feature/misc feature/cds CDS feature/start start_codon feature/stop stop_codon feature/s_mutation point_mutation feature/s_mutation point_mutation feature/misc sequence_feature |