Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Vuong
Date created: 2011-09-22 11:00:00
Date modified: 2015-05-08 01:12:39
pTet Regulated Arg-tagged ECFP and EYFP (FRET Reporter)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K542005_sequence (Version 1) |
Description
Made by assembling BBa_K331033 with BBa_K331035.The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP and Arg-tagged EYFP will be produced constitutively. By placing this part downstream of BBa_K542003, expression of the fluorescence proteins maybe "turned off" in the presence of arabinose.
Fluorescence/F??rster Resonance Energy Transfer(FRET) is a distance-dependent phenomenon in which the excitation of a donor fluorophore leads to emission by an acceptor fluorophore; this occurs if the two fluorophores are within a certain distance to each other. The distance is dependent on the FRET pair used. The emission spectrum of the donor fluorophore must overlap with the excitation spectrum of the acceptor fluorophore for FRET to occur. FRET is explained in further detail on the Lethbridge 2009 Wiki. This phenomenon will allow for characterizing co-localization within the Lumazine Synthase microcompartment.
Notes
In order to characterize the Lumazine Synthase microcompartment, the fluorescent proteins must be regulated by a different promoter than that of Lumazine.| Access | Instance | Definition |
| public public | BBa_K331033 BBa_K331035 | BBa_K331033 BBa_K331035 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K331033 BBa_K331035 | 1,836 845,1755 | BBa_K331033 BBa_K331035 |