BBa_K545042

BBa_K545042 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K545042
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grenoble 2011
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:39

Toggle Switch (LacI - TetR) which express GFP in one side



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K545042_sequence (Version 1)

Description

it's a Toggle Switch. Two repressors inhibits the other. the TetR side express the GFP too.

We have pTet promoter that regulate LacI gene, follow by two terminators and finally pLac promoter regulate TetR and GFP genes.

TetR repress the pTet promoter and be inhibited by (anhydro-)tetracycline.
LacI repress the pLac promoter and be inhibited by IPTG (or Lactose)

Notes

the sesquencing of this Toggle Switch part is conform to the original sequences.

Source

this composite part is an assembly from diverse parts (R0040, Q04121, B0034, C0040, E0240)

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K545042/1