Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laurynas VANAGAS
Date created: 2011-10-01 11:00:00
Date modified: 2015-05-08 01:12:39
ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K547000_sequence (Version 1) |
Description
ready-to-inject backbone for T3SS. Used for protein assembly and expression.Notes
Removed Spe1 restriction site from GSK tag by silent mutation.Source
SlrP: S. enterica TyphimuriumGSK: Glycogen Synthase Kinase 3beta
Julie Torruellas Garcia, Franco Ferracci, Michael W. Jackson,1 Sabrina S. Joseph, Isabelle Pattis, Lisa R. W. Plano, Wolfgang Fischer, and Gregory V. Plano. 2006. Measurement of Effector Protein Injection by Type III and Type IV Secretion Systems by Using a 13-Residue Phosphorylatable Glycogen Synthase Kinase Tag. Infect Immun.Vol.74:5645-57.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0015 Biobrick Suffix E. coli his operon terminator Stem loop rep (pMB1) Kanamycin resistance marker G ->C Silent mutation repeat regeion End of terminator Stem loop Biobrick Prefix BBa_R0040 BBa_K496003 BBa_B0034 SlrP mat_peputide T -> A Silent mutation BsaI Cloning Site BBa_E0040 BsaI Cloning Site BsaI Cloning Site pSB1K3 | 8,136 137,157 158,229 166,200 397,1011 1233,2048 2016,2016 2153,2196 2279,2282 2292,2311 2319,2340 2341,2394 2402,2988 2402,2413 2416,2988 2416,3729 2586,2586 2991,3001 3010,3729 3732,3735 1,7 157,2319 | feature/BioBrick engineered_region sequence_feature feature/misc stem_loop feature/stem_loop stem_loop feature/stem_loop sequence_feature feature/misc feature/cds CDS feature/s_mutation point_mutation feature/misc sequence_feature feature/stem_loop stem_loop feature/stem_loop stem_loop feature/misc sequence_feature engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region feature/misc sequence_feature feature/protein CDS feature/s_mutation point_mutation feature/misc sequence_feature feature/BioBrick engineered_region sequence_feature feature/misc sequence_feature feature/misc engineered_region feature/BioBrick |