BBa_K549002

BBa_K549002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K549002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sabine Wagner

Date created: 2011-09-11 11:00:00

Date modified: 2015-05-08 01:12:39

Nickel(II) dependent promotor with lacZ' as reporter

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_K549002_sequence (Version 1)

Description

The biobrick contains the RcnA promotor from E. coli fused with the lacZ' reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the LacZ' is expressed.

Notes

There where no special considerations we had to deal with.

Source

The RcnA promotor is amplified from E. coli K12
The lacZ' reporter gene is abstracted from the BB_J33202.

Components

Access	Instance	Definition
public	BBa_K549038	BBa_K549038
public	BBa_J33202	BBa_J33202

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K549038	1,213	BBa_K549038
BBa_J33202	222,464	BBa_J33202

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K549002/1

Member of these Collections

• iGEM Parts Registry