Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sabine Wagner
Date created: 2011-09-11 11:00:00
Date modified: 2015-05-08 01:12:39
Nickel(II) dependent promotor with luxAB as reporter
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K549003_sequence (Version 1) |
Description
The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.Notes
There where no special considerations we had to deal with.Source
The RcnA promotor is amplified from E. coli K12The luxAB reporter gene is abstracted from the BB_K216008.
| Access | Instance | Definition |
| public public | BBa_K549038 BBa_K216008 | BBa_K549038 BBa_K216008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K549038 BBa_K216008 | 1,213 222,2331 | BBa_K549038 BBa_K216008 |