BBa_K561003

BBa_K561003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K561003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: MA Keyue

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:41

PfdhF+RFP+tetR+Pvgb+YFP+tetR+Ptet+CFP

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K561003_sequence (Version 1)

Description

This device expresses RFP,YFP,CFP respectively under different oxygen concentration. RFP is expressed under anaerobic conditions. YFP is expressed under microaerobic conditions. CFP is expressed under aerobic conditions and is repressed with the existence of TetR.

Notes

We construct tetR to resist CFP expression under microaerobic and anaerobic conditions.

Source

vgb promoter comes from Vitreoscilla sp. and fdhF comes from E.coli.

Components

Access	Instance	Definition
public	BBa_K561002	BBa_K561002
public	BBa_K561000	BBa_K561000
public	BBa_I13602	BBa_I13602

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K561002	1,1679	BBa_K561002
BBa_K561000	1688,3420	BBa_K561000
BBa_I13602	3429,4407	BBa_I13602

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K561003/1

Member of these Collections

• iGEM Parts Registry