Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduABTUN BMC Components
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K562008_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA, PduB, PduT, and PduU proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562007) AND protein PduN. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB, PduU and PduN gene products carry C-terminal 6-His affinity/epitope tags. Production of the PduB, PduU and PduN proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT, PduU and PduN have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTUN in the Sargent Laboratory, Dundee, UK.Notes
Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3'end.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562007 synthetic RBS pduA gene pduB gene Hexa-Histidine Tag synthetic RBS pduT gene pduU gene Hexa-Histidine Tag synthetic RBS pduN gene Hexa-Histidine Tag | 1,2180 107,112 119,403 400,1209 1210,1227 1240,1245 1252,1806 1806,2153 2154,2171 2184,2189 2196,2468 2469,2486 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs CDS feature/cds CDS feature/cds sequence_feature feature/misc ribosome_entry_site feature/rbs CDS feature/cds feature/cds CDS feature/misc sequence_feature ribosome_entry_site feature/rbs CDS feature/cds sequence_feature feature/misc |