Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guo Jing
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
pEASY+33Tor2(with E->K mutation)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K563037_sequence (Version 1) |
Description
There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid E to amino acid K.Notes
We will use overlap PCR to conduct this mutation. After we get this mutant part of Tor2, we can keep it for further connection.Source
pEASY vector is bought from Transgene Company, and we get mutant-33Tor2 by mutating the 33Tor2 from genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| 33Tor2 (EK mutation) Kanamycin resistance ORF Ampicillin Resistance ORF EK mutation site | 294,2792 3816,4610 4628,5488 2333,2335 | CDS feature/cds CDS feature/cds feature/cds CDS sequence_feature feature/dna |