Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Damian Rafal Plichta
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42
Regulatory device based on sRNA
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K564007_sequence (Version 1) |
Description
This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5??? untranslated region (UTR) near ribosome binding site (RBS) of chip messenger RNA (mRNA). (Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target. Figueroa-Bossi et al. 2009, Genes and development 23:2004-2015) This leads to degradation of the targeted mRNA by enzymes called RNases. (Lauren S. Waters and Gisela Storz, Regulatory RNAs in Bacteria, Cell 136, 615-628, 2009) The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chip gene transcription. (Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target. Figueroa-Bossi et al. 2009, Genes and development 23:2004-2015) It is fused with Ptet promoter.Notes
No design considerationsSource
Escherichia coli w3110 genomic DNA| Access | Instance | Definition |
| public public public public | BBa_R0040 BBa_K564000 BBa_B0010 BBa_B0012 | p(tetR) BBa_K564000 BBa_B0010 BBa_B0012 |