Ptet-lacZ

BBa_K564008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K564008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Damian Rafal Plichta
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42

Ptet fused with LacZ



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Measurement

    Sequences BBa_K564008_sequence (Version 1)

    Description

    Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog,aTc.. In this part it is fused with lacZ which encodes β-galactosidase (an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose). This fusion allows us to check if Ptet works correctly using β-galactoassays.

    Notes

    No design considerations

    Source

    Escherichia coli w3110 genomic DNA

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K564008/1
     

    Attachments

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