Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Lewinska
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42
atC-->chiX
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K564014_sequence (Version 1) |
Description
This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5??? untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases.The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter.Notes
There are no design considerationsSource
The subparts were obtained from minigenes and fused together.| Access | Instance | Definition |
| public public public public | BBa_K564021 BBa_K564000 BBa_B0010 BBa_B0012 | BBa_K564021 BBa_K564000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K564021 BBa_K564000 BBa_B0010 BBa_B0012 | 1,925 934,1017 1026,1105 1114,1154 | BBa_K564021 BBa_K564000 BBa_B0010 BBa_B0012 |