Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Lewinska
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42
Mutated regulatory device based on sRNA.
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K564020_sequence (Version 1) |
Description
This device is based on gene encoding small RNA (sRNA)called ChiX, which represses ChiP gene synthesis by pairing with a sequence in 5??? untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases. The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter. In this biobrick we introduced mutations in the ChiP and ChiX binding site of ChiX in order to show that basepairing is essential for this regulatory system.Notes
There are no design considerations.Source
Both promoter as well as ChiX was obtained from minigenes.| Access | Instance | Definition |
| public public public public | BBa_K564018 BBa_K564019 BBa_B0010 BBa_B0012 | BBa_K564018 ChiXm BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K564018 BBa_K564019 BBa_B0010 BBa_B0012 | 1,624 633,716 725,804 813,853 | BBa_K564018 ChiXm BBa_B0010 BBa_B0012 |