Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez and Porfirio Quintero
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43
penI-pCpcG2-pPenI-mnt
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K566006_sequence (Version 1) |
Description
Potential and gate.Composed of pCpcG2 promoter which may be inducible by green light, this way it express penI and this represses mnt expression, allowing the transcription of any gene controlled under pMnt promoter.
This construction may be used with another one which represses mnt expression with red light and this way it may act as an and-gate.
Notes
Search for illegal sites was performed, they were found and removed.Source
Sequence of pCpcG2 promoter was obtained from pJT122 plasmid of Dr. Jeff Tabor. Rest of the sequences were obtained from the Registry.Complete construction was synthesized.
| Access | Instance | Definition |
| public public public public public public | BBa_K566010 BBa_B0034 BBa_K566009 BBa_R0074 BBa_B0034 BBa_K566008 | PenI inv BBa_B0034 pCpcG2 inv PenI BBa_B0034 Mnt opt |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K566010 BBa_B0034 BBa_K566009 BBa_R0074 BBa_B0034 BBa_K566008 | 1,423 424,435 436,675 676,752 753,764 765,1052 | PenI inv BBa_B0034 pCpcG2 inv PenI BBa_B0034 Mnt opt |