Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:43
E. coli MXGreen
| Types | DnaRegion |
| Roles | chromosome Cell |
| Sequences | BBa_K566031_sequence (Version 1) |
Description
Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.It is proposed as a photo-chassis that could make useful tools in this field of light induction.
Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.
Notes
UANLBricks for assambly of photochassis. Dr. Kuhlman integration protocol.Source
JT2 strain, genes from BioBricks and Dr. Jeff Tabor plasmids| Access | Instance | Definition |
| public public public public | BBa_K566023 BBa_K566024 BBa_K566029 BBa_K566030 | BBa_K566023 BBa_K566024 BBa_K566029 BBa_K566030 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K566023 BBa_K566024 BBa_K566029 BBa_K566030 | 1,948 957,1880 1889,2794 2803,5265 | BBa_K566023 BBa_K566024 BBa_K566029 BBa_K566030 |